Detailed view for PF3D7_0715300

Basic information

TDR Targets ID: 829677
Plasmodium falciparum, calcium/calmodulin-dependent protein kinase, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.9986 | Length (AA): 517 | MW (Da): 61331 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 82 2z7x (B) 445 526 6.00 0.5 0.01 0.0254046 0.58
86 512 4yhj (A) 62 451 20.00 0 1 0.768119 0.99
248 511 2hw6 (A) 72 334 36.00 0 0.99 0.418938 1
423 510 4ks7 (A) 565 658 24.00 0.00024 0.71 0.469813 -0.89

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs. Otto TD
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 32 hs, Oocyst, Female Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, Ring. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 24 hs, Sporozoite, Male Gametocyte. Otto TD Zanghi G Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_154761)

Species Accession Gene Product
Neospora caninum NCLIV_025300   CAM kinase, putative
Plasmodium berghei PBANKA_1421600   calcium/calmodulin-dependent protein kinase, putative
Plasmodium falciparum PF3D7_0715300   calcium/calmodulin-dependent protein kinase, putative
Plasmodium knowlesi PKNH_1424300   calcium/calmodulin-dependent protein kinase, putative
Plasmodium yoelii PY02877   serine/threonine-protein kinase
Toxoplasma gondii TGME49_262540   protein kinase, putative

Essentiality

PF3D7_0715300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1421600 Plasmodium berghei Dispensable plasmo
TGME49_262540 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 20.9% 330 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 19.8% 278 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 22.5% 306 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 23.8% 307 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 20.4% 285 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 19.7% 289 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 18.2% 203 aa Compounds References
Patiria pectinifera Cdc2 300 aa 20.0% 290 aa Compounds References
Oryctolagus cuniculus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 22.0% 282 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0003 0.5 0.5
0.0027 1 0.5
0.0016 0.5 0.5
0.0061 0.6883 1
0.0033 1 1
0.0007 0.5 0.5
0.0029 0.5 0.5
0.0037 1 0.5
0.0056 1 0.5
0.0023 0.5 0.5
0.0066 0.3101 0
0.0091 1 0.5
0.0026 0.5 0.5
0.0039 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0059 1 1
0.0059 1 1
0.0081 1 0.5
0.0012 0.5 0.5
0.0036 0.5 0.5
0.0092 1 0.5
0.0093 0.8828 0
0.0067 0.5 0.5
0.0042 0.5 0.5
0.0039 0.9485 0.5
0.0032 0.5 0.5
0.0069 0.3067 1
0.0088 0.4477 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0064 0.3377 0
0.0018 0.5 0.5
0.0059 1 1
0.0039 0.5 0.5
0.0063 0.7244 0.7244
0.0022 0.5 0.5
0.0063 1 1
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0062 0.6935 0
0.0032 0.5 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0098 0.3242 0.2614
0.0011 1 0.5
0.0008 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_0715300 (Plasmodium falciparum), calcium/calmodulin-dependent protein kinase, putative
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